Equilibrium and rapid kinetic studies on nocodazole-tubulin interaction.
نویسندگان
چکیده
منابع مشابه
Equilibrium and rapid kinetic studies on nocodazole-tubulin interaction.
The interaction between nocodazole and calf brain tubulin in 10(-2) M sodium phosphate, 10(-4) M GTP, and 12% (v/v) dimethyl sulfoxide at pH 7.0 was studied. The number of binding sites for nocodazole was shown to be one per tubulin monomer of 50,000 as a result of equilibrium binding studies by gel filtration and spectroscopic techniques. The presence of microtubule-associated proteins did not...
متن کاملEquilibrium Modeling and Kinetic Studies on the Adsorption of Basic Dye by a Low Cost Adsorbent
An agricultural waste and eco-friendly biosorbent i.e. rice husk has been used as a cheap adsorbentfor the removal of methylene blue dye from aqueous solutions. The physical properties of the developedadsorbent were characterized using FTIR. The study was realized using batch experiments. The effectsof contact time, pH, initial dye concentration, biosorbent dose and temperature were investigate...
متن کاملEquilibrium and Kinetic Studies on Lead (II) Adsorption by Sugarcane Bagasse Derived Activated Carbon (TECHNICAL NOTE)
Municipal and industrial wastewater contain a lot of contaminants. The major contaminants of concern are heavy metals. Heavy metals are known to be toxic, non-biodegradable and have a long half-life. The release of untreated wastewater containing heavy metals can cause serious problems to human, plants and animals. In this study, activated carbon was developed from sugarcane bagasse and its eff...
متن کاملEquilibrium and Kinetic Studies on the Adsorption of Acid Yellow 36 Dye by Pinecone
Background & Aims of the Study: Dyes have significant role in environmental problems, due to their toxic effects on the food chain and sources of water. The purpose of this research was to study the adsorption of acid yellow 36 dye onto pinecone using batch system. Materials & Methods: This research was performed at laboratory scale and batch system. Equilibrium isotherms...
متن کاملKinetic and equilibrium studies on crystalline 1-glutamic acid dehydrogenase.
The spectrophotometric procedure for determining the specific activity of the enzyme and the preparation of reagents employed in the assay have been described previously (3). DPiV-Commercial preparations of DPN (60 per cent) were used in routine assays. For critical experiments, commercial DPN was further purified by several adsorptions on Dowex 1 formate columns by the method of Kornberg and P...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)39148-2